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KMID : 0358219940210010063
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Korean Journal of Fertility and Sterility
1994 Volume.21 No. 1 p.63 ~ p.68
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Evaluation of Water Quality using ICR Mouse 1-cell Emnryo
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김충현
정경순/박소현/황도영/김기철/민응기
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Abstract
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To confirm the overcome of in vitro 2-cell block, ICR mouse 1-cell embryos were cultured in CZB media. All embryos in CZB were overcome in vitro 2-cell block and 92% of embryos were developed to the blastocyst at day 4. However, in m-KRB group
(control)
only 20% of embryos were developed over 2-cell. Any embryos in m-KRB did not develop to the morular stage.
Developments and degenerations of ICR mouse 1-cell embryos were compared in CZB medium prepared with water of three quality: (1) Milli-Q ultrafiltration water (UF); (2) Milli-Q reverse osmosis water (RO); (3) tap water (TAP). The objective was to
evaluate the potential of quality control using ICR mouse 1-cell embryos. The more water was purified, the better embryos developments were supported and the less embryos were degenerated.
As a quality control system, the culture of ICR 1-cell mouse embryos in CZB was useful.
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